FastQCFastQC Report
Fri 10 Feb 2017
SRR3559657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727690
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469321.720576751756981No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238890.8757960032115087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143970.5278092451854866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89590.3284464143652688No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88630.3249269528428817No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56630.20761156876331252No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42390.15540622284790426No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.1351693190941786No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36330.13318962198783585No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30290.11104634324281717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA217650.050.6102641
TAACGCA4800.049.5826154
TACAACG5250.047.6036762
TATCACG3750.046.0168842
CGCATAG5650.043.231051
ATCAACG276650.039.465363
ACAACGC6350.039.353763
TATCAAC278100.039.3487742
TCAACGC282500.039.3220864
CAACGCA285650.039.1175845
AACGCAG294650.038.790346
ACGCAGA323200.035.3637857
CGCAGAG328550.034.8603678
CATGGGT42650.033.7603574
GCAGAGT343500.033.3258369
GTATAAC9100.031.423911
GTACAAC10550.029.9284171
AGTACTT207750.028.38149812-13
ACATGGG220400.027.9948313
GGTATCA125250.027.8727761