FastQCFastQC Report
Fri 10 Feb 2017
SRR3559656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905515
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532392.7939428448477184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257311.3503436078960283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161440.8472250284043947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99050.5198069813147627No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88300.46339178647242346No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60770.31891640842501895No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44770.23494960679921176No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42570.22340417157566328No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41010.21521740841714707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34180.17937407997313062No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT28000.1469419028451626No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24050.12621259869379145No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23720.12448078341025916No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC23650.12411342865314627No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21210.11130849140521067No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21050.1104688233889526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4250.056.0726931
GTATCAA226800.052.6160471
TACAACG3900.042.7240072
TATCAAC284350.041.813912
ATCAACG289050.041.1459583
TCAACGC296650.040.6734474
AACGCAG307400.040.2187966
CAACGCA301200.040.1380355
ACGCAGA337100.036.710647
CGCAGAG340950.036.5229538
ATAGTAC6950.035.9543844
TAACGCA5050.035.3441284
GCAGAGT353350.035.1907549
ACAACGC4750.035.0712973
CATGGGT32300.033.708234
GGTTTCA10700.032.8509981
GTACAAC6250.031.456781
TATCACG3700.030.5583882
TCACGCA4200.029.7479724
GTATAAC6150.029.0620671