FastQCFastQC Report
Fri 10 Feb 2017
SRR3559654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282146
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT563971.7182965047868073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264240.8050830158073407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171740.5232552116816254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102090.3110464921426408No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83210.2535231522302786No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59620.18164944521054213No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.1261369847654553No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40060.12205429008947195No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35260.10742971214565106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA236700.059.2836071
TATCAAC301900.046.181542
ATCAACG302550.045.9390533
TCAACGC307650.045.815724
CAACGCA313500.045.093635
AACGCAG324850.044.3056766
ACGCAGA357400.040.2372677
CGCAGAG364000.039.6215068
GCAGAGT381850.037.7849359
TAACGCA6050.034.4206474
GGTATCA119000.034.149111
CGCATAG5100.032.713711
TACAACG6600.032.4577062
CATGGGG103100.030.1820964
ACAACGC7150.029.9573123
AGAGTAC371500.029.19628310-11
AGTACTT231150.029.0860212-13
CATGGGT48050.028.7277054
ACATGGG246500.028.409653
GTACATG264750.027.862921