FastQCFastQC Report
Fri 10 Feb 2017
SRR3559653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143572
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382491.7843580714806875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176120.8216192411544843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122930.5734820197315509No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59700.27850709003476437No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35600.1660779297359734No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34370.1603398439613878No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.13146281067302615No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27980.13052978859585776No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22420.10459177485057651No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22060.1029123351116734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA189900.054.7052841
CGCATAG3850.052.604871
TATCACG2800.046.7515532
TATCAAC235800.043.7054372
ATCAACG237900.043.3426253
TCAACGC242050.043.1658824
CAACGCA245900.042.5858425
AACGCAG251000.042.5739256
TAACGCA3250.042.1081054
ACGCAGA276500.038.5830237
CGCAGAG281350.037.9602138
GCAGAGT292650.036.4131439
GGTATCA102800.032.1593781
TACAACG4650.030.7106932
CATGGGG68150.030.64524
CATGGGT28400.028.2837264
AGAGTAC286100.027.93996210-11
AGTACTT182600.027.5169212-13
ACATGGG181550.027.267053
GAGTACT164500.026.83730912-13