FastQCFastQC Report
Fri 10 Feb 2017
SRR3559652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3664217
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT735892.0083144639086603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361130.9855584426359029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237570.648351339453968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116580.31815801302160873No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA90180.24610987831779613No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70130.19139150328705967No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65930.17992930003872587No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT57970.15820569578712176No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54020.14742576654166498No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53120.14496958013130773No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51430.14035740787185913No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC49120.13405319608527552No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.12499259732706879No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41200.11243875567413175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40150.1095732048620483No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT39710.10837240261698475No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA38250.104387922440183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA386100.049.21791
CGCATAG7600.041.542681
TAACGCA6050.040.321154
TATCAAC474750.039.8453252
ATCAACG481300.039.2616923
TCAACGC490350.038.901094
CAACGCA492800.038.707695
AACGCAG504800.038.247216
ACGCAGA547700.035.2296757
CGCAGAG555250.034.868518
TATCACG5950.034.002732
GCAGAGT578650.033.386499
TACAACG6300.033.0582122
GTATAAC9800.032.2167661
TACTGGT29950.029.4046972
GTACTGG35000.028.7641011
CATGGGT36000.028.4269584
CATGGGG114000.028.0790144
GAGTACT315350.027.04601912-13
AGTACTT364150.026.97523512-13
Produced by FastQC (version 0.11.5)