FastQCFastQC Report
Fri 10 Feb 2017
SRR3559649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3935229
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453791.153147631306844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218140.5543260633625133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143160.36379077304014584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68750.1747039371787512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA226550.058.453331
ATCAACG296600.044.2319153
TCAACGC300300.044.2229964
TATCAAC300500.043.8388332
CAACGCA304150.043.7218975
AACGCAG315600.042.9086236
ACGCAGA351800.038.3918767
CGCAGAG361850.037.4406858
GCAGAGT378450.035.7040869
TATCACG5500.034.6188932
GGTATCA116250.033.5134121
CGCATAG6200.029.7854291
TAACGCA4600.029.7494774
AGAGTAC367250.028.73248310-11
GTACATG274950.027.3642441
ACATGGG259550.027.2099673
CATGGGG114900.027.0826364
AGTACTT228550.026.8786312-13
TACATGG278150.026.8680692
GTATATA16050.025.6099011
Produced by FastQC (version 0.11.5)