FastQCFastQC Report
Fri 10 Feb 2017
SRR3559646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6262872
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1566112.500625910923934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT743021.1863886089321323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455550.7273819423421076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266370.42531605308235587No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178420.2848852730823814No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143120.22852135569751386No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120390.19222810237858923No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118620.18940192295164263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA470150.071.522791
TATCAAC623950.053.7905732
ATCAACG631450.053.019433
TCAACGC643400.052.3865054
CAACGCA651350.051.8110475
AACGCAG665900.051.1614536
GGTATCA186400.050.7572941
ACGCAGA736000.046.3047757
CGCAGAG750550.045.4388288
GCAGAGT799750.042.5988279
AGAGTAC778400.032.80618310-11
AGTACTT580550.030.89402412-13
GTACATG428100.029.0310651
ACATGGG415050.028.8706233
GAGTACT542850.028.52395412-13
TACATGG433550.028.494762
CAGAGTA788600.027.90407810-11
CATGGGG180450.027.8942094
TACTTTT667150.026.94633314-15
CGCATAG9650.025.9307961
Produced by FastQC (version 0.11.5)