FastQCFastQC Report
Fri 10 Feb 2017
SRR3559645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775421
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503802.837636819661365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246741.3897548806733728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160940.9064892214297342No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92350.5201583173793709No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66340.3736578535457224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50120.28229924057448913No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT37570.2116117810930478No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.20316308075662054No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34120.19217977031926514No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29280.16491863056705985No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC27290.1537100214540664No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT25320.14261406167889193No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA24460.13777014015267364No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22810.1284765697826037No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA18430.10380636480023611No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT18360.1034120921178695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA174700.064.054551
TATCAAC227100.049.1338272
ATCAACG230150.048.4717673
TCAACGC234850.048.0590634
CAACGCA237350.047.8536725
AACGCAG242300.047.489946
GGTATCA81700.046.8231121
ACGCAGA268600.042.795667
CGCAGAG271650.042.3808638
GCAGAGT283800.040.5664639
TAACGCA2800.038.248384
CGCATAG3500.035.751851
GACCGTT4150.034.408187
TATAGCG1051.4845695E-533.998565
TACTGGT14000.033.1560672
GTACTGG17050.031.8027251
AGAGTAC279700.031.22713710-11
TACAACG3300.030.6571222
ACGGTAC5700.030.2706473
AGTACTT202700.029.26442112-13
Produced by FastQC (version 0.11.5)