FastQCFastQC Report
Fri 10 Feb 2017
SRR3559644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786423
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT786572.077343181150125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381071.0064115921543895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238970.6311233583780786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145920.38537691113750366No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124480.3287535491940547No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91040.24043800705837673No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70880.18719514433543216No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65070.17185084709236131No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60050.15859295171194557No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52550.1387853390918025No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT39190.10350137847778762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA323250.055.130351
TATCAAC415500.042.866182
ATCAACG418450.042.4713633
TCAACGC428350.042.073154
CGCATAG6700.041.7961161
CAACGCA433200.041.7119875
AACGCAG442950.041.7066886
TACAACG6450.039.6723442
ACGCAGA486800.037.8887187
CGCAGAG496650.037.3404358
GCAGAGT516550.035.8788729
CATGGGT72900.033.2994584
TATCACG4950.032.459192
CATGGGG119850.031.3253444
GGTATCA173300.031.3207911
ACATGGG338200.028.8695163
GTACATG367600.028.3807261
AGTACTT309200.028.31482312-13
TACATGG369400.028.1271972
AGAGTAC508000.027.92715510-11