FastQCFastQC Report
Fri 10 Feb 2017
SRR3559641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990266
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435432.187798012928925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209071.05046260148141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143850.7227677104467444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80190.40291096767969714No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74970.3766833177072813No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.24112354830962296No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.16711334062884056No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.16228986477184457No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29340.14741748087944023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171800.058.978461
TAACGCA4450.050.807384
TATCAAC220600.045.749782
ATCAACG223300.045.216443
TCAACGC229500.044.798584
CAACGCA232750.044.19865
AACGCAG239700.043.5861786
CGCATAG4900.041.3571361
ACGCAGA264250.039.559347
CGCAGAG268450.039.1177258
GCAGAGT279750.037.6014379
GGTATCA85850.035.96311
GTATAAC7650.033.5023041
TACAACG3800.032.8854982
ATAACGC9200.031.6892153
TATCACG2850.031.3195192
GGACCGT5800.030.7741156
AGAGTAC274800.029.80243910-11
AGTACTT182150.029.0542112-13
ACAACGC4550.028.768323
Produced by FastQC (version 0.11.5)