FastQCFastQC Report
Fri 10 Feb 2017
SRR3559639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422670
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT762741.406576465099296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370900.6839804007988685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222830.4109230323807275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143880.2653305474978193No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125420.23128827680828817No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92330.17026667674780138No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59950.11055439479075806No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58490.10786199418367705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA302550.058.1518481
TCAACGC397450.044.175644
ATCAACG395200.044.0357173
TATCAAC397500.043.950432
CAACGCA404500.043.523385
AACGCAG418100.042.804936
ACGCAGA464100.038.4981547
CGCAGAG476100.037.5902988
GCAGAGT507800.035.2671179
GGTATCA153400.033.596031
CGCATAG9950.030.5382631
CATGGGG154400.029.0549534
GTACATG350300.028.8968471
AGAGTAC485900.028.63430210-11
ACATGGG331400.028.4918443
TACATGG358050.028.0858292
TAACGCA7650.027.9986554
AGTACTT319250.027.32141712-13
GAGTACT287650.025.20352712-13
ACAACGC11250.024.3277233
Produced by FastQC (version 0.11.5)