FastQCFastQC Report
Fri 10 Feb 2017
SRR3559638.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559638.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4782998
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1086412.2713996535227485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518901.084884417681128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328070.6859087124853491No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194830.40733866081482784No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161950.3385951656262453No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122030.25513286854813655No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90370.18894007482336392No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82230.17192146013859927No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76910.1607987291652641No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63230.13219742094811665No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC57190.1195693579633527No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT55570.11618236093763785No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51240.10712946148001734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA439700.056.898381
TATCAAC558800.044.639682
ATCAACG566650.043.9107483
TCAACGC576300.043.6503874
CAACGCA583600.043.206335
AACGCAG594500.042.8545076
ACGCAGA652650.038.9268467
CGCAGAG661500.038.4240468
GCAGAGT690700.036.816869
CGCATAG8150.033.628751
GGTATCA230750.033.024731
TATCACG7550.032.3141752
CATGGGT72250.031.3753684
CATGGGG149950.029.7588754
AGTACTT441150.028.62631212-13
GACCGTT8950.028.5855877
AGAGTAC677550.028.57445110-11
TAACGCA6550.028.1593154
GAGTACT392300.027.83812912-13
ACATGGG411400.027.5072983
Produced by FastQC (version 0.11.5)