FastQCFastQC Report
Fri 10 Feb 2017
SRR3559637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6569521
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1099141.6730900167607348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523350.796633422741171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310700.4729416345575271No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198190.3016810510233547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161040.2451320271295274No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.18298137718107607No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86550.13174476495318305No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85040.12944627165359543No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77370.11777114343648493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA447000.057.738391
TATCAAC581750.044.231022
ATCAACG581200.044.1218073
TCAACGC588950.044.076974
CAACGCA596500.043.6088525
AACGCAG611950.043.139836
ACGCAGA678500.038.934817
CGCAGAG693700.038.1846168
CGCATAG10350.037.415951
GCAGAGT731350.036.2188649
TATCACG9300.033.2734682
GGTATCA229100.031.440181
CATGGGG204150.028.7072624
AGAGTAC710000.028.65106210-11
AGTACTT442600.028.31707412-13
ACATGGG479150.028.063293
GTACATG510650.027.9542141
TACATGG518000.027.3301722
TAACGCA7850.027.2858684
CATGGGT85750.027.1992474