FastQCFastQC Report
Fri 10 Feb 2017
SRR3559636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5577416
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1413772.534811819667029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT682061.2228960507876765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418410.7501861076885784No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255050.45729061629973444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222840.39953985860118735No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162180.29077981631637306No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA133420.23921471878733808No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116360.20862707748534448No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT108830.19512620181101786No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107040.1919168303027782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA556850.055.213581
TATCAAC704650.043.5493242
ATCAACG714250.042.8506933
TCAACGC727050.042.628224
CAACGCA736650.042.0403825
AACGCAG749600.041.8527156
CGCATAG8550.038.3293081
ACGCAGA821000.038.1331867
CGCAGAG830300.037.7562268
GCAGAGT861550.036.3660249
TACAACG10900.031.663522
GGTATCA297200.031.5165121
TAACGCA8700.031.4590574
TATCACG7400.031.3610532
CATGGGT81300.030.9568634
CATGGGG169200.030.1009714
AGTACTT545300.029.59031912-13
AGAGTAC849850.028.23458110-11
GAGTACT503700.027.4215612-13
ACAACGC13500.026.4438443