FastQCFastQC Report
Fri 10 Feb 2017
SRR3559634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434953
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1337302.0781814568031813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT645761.0035193730241696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402570.6255989748487674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235650.3662031408776412No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174970.2719056378500356No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133260.2070877596153383No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105240.16354431803930813No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103730.1611977585539475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA456900.067.099871
TATCAAC607300.050.2867052
ATCAACG614450.049.5679443
TCAACGC626500.049.3553164
CAACGCA633950.048.794085
AACGCAG652000.048.191556
GGTATCA198450.044.8297351
ACGCAGA720650.043.5347147
CGCAGAG735400.042.7501958
GCAGAGT779650.040.2475479
TATCACG8950.031.9144972
AGAGTAC761100.031.77320310-11
CGCATAG12700.030.0285031
CATGGGT85350.029.9058444
ACATGGG434200.029.8175093
GTACATG452900.029.8136441
AGTACTT557850.029.46334812-13
TACATGG458850.029.3872532
CATGGGG183350.028.7186934
TAACGCA8750.028.5590344
Produced by FastQC (version 0.11.5)