FastQCFastQC Report
Fri 10 Feb 2017
SRR3559632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527359
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830162.353488828327369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405541.1496986839162104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256110.7260672928386366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159350.451754414563417No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124300.3523882882349089No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93420.26484403770639736No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69770.19779670852895895No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67180.19045410461481238No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67090.19019895621625132No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53210.15084940319372084No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC50720.14379029750019773No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT48250.1367878914507993No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39270.11132975123881635No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37090.10514949002922583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA360600.055.774041
CGCATAG5550.049.3764951
TATCAAC456550.043.973872
ATCAACG465650.043.0213973
TCAACGC478350.042.4762234
CAACGCA483800.042.1084145
AACGCAG497400.041.650866
TAACGCA5700.040.709034
ACGCAGA546800.037.8226747
CGCAGAG553150.037.4637768
GCAGAGT579000.035.7397889
TTAGGAC11000.032.453353
GTATAAC9000.032.434631
AGTGTAC13450.031.8501283
TATCACG7100.031.8466052
GGTATCA193000.031.7006761
TACTGGT25150.030.756952
TACAACG6850.030.4029242
AGAGTAC566400.028.42533110-11
AGTACTT383400.028.16602112-13