FastQCFastQC Report
Fri 10 Feb 2017
SRR3559631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369456
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT768882.2819113827276567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354171.0511192311162396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231060.6857486787184637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130480.38724351942865554No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97320.2888300069803553No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75400.22377499513274546No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58280.17296560631745897No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.17163007915817866No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43490.12907128034911275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA276350.064.1685641
TATCAAC359850.049.2460672
ATCAACG360550.049.192673
TCAACGC368150.048.6943134
CAACGCA372550.048.119215
AACGCAG382200.047.4951176
ACGCAGA423000.042.857777
GGTATCA126950.042.7127531
CGCAGAG432250.042.036988
GCAGAGT452750.040.19939
TATCACG5100.036.1709482
TAACGCA4200.031.165734
TACAACG6900.031.047152
CATGGGG100950.030.824734
AGAGTAC444000.030.76038710-11
ACATGGG251050.028.889993
GTACATG264800.028.7582021
AGTACTT322750.028.75802212-13
TACATGG267300.028.3621272
GAGTACT294450.027.66257512-13
Produced by FastQC (version 0.11.5)