FastQCFastQC Report
Fri 10 Feb 2017
SRR3559630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630549
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT941372.592913633723164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455521.2546862747204348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285800.7872087664978492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164460.4529893412814426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136920.37713304516754903No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98400.2710333891651097No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76310.21018859682103175No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73780.20321995378660362No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69450.19129338290159423No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT56830.1565327998602966No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC46590.12832769919921203No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT46510.1281073468502973No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG38740.10670562496195479No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC36780.10130699241354406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA389200.054.637851
CGCATAG6650.043.0070341
TATCAAC494900.042.9758262
ATCAACG501950.042.221233
TCAACGC515150.041.6590964
CAACGCA519850.041.362575
AACGCAG530950.041.271056
TACAACG5700.040.7171062
ACGCAGA583400.037.5912137
CGCAGAG590100.037.2344638
GCAGAGT615000.035.6785559
TAACGCA5700.032.3581924
GGTATCA212250.030.79491
TATCACG6650.029.5310882
AGTACTT390600.029.40552912-13
GACCGTT8100.029.3813937
CATGGGG112550.028.8632074
CATGGGT49050.028.505344
AGAGTAC603550.028.07982610-11
GAGTACT358750.027.58813312-13