FastQCFastQC Report
Fri 10 Feb 2017
SRR3559629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348541
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569902.4266129482091223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265651.1311277938090074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184560.7858495976863934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97840.41659907150865155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80770.3439156480555375No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54750.23312345835137643No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41220.17551322289029658No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37240.15856653130603213No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31970.13612706782636538No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31890.13578643080959626No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC30110.12820725718648301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA248550.055.6838041
CGCATAG4700.051.995581
TAACGCA4050.047.009844
TATCAAC308300.044.8551032
AACGCAG317850.044.7756586
ATCAACG314400.043.9224363
TCAACGC322050.043.3594324
CAACGCA325050.043.0690775
ACGCAGA349350.040.636177
CGCAGAG354300.040.1691938
GCAGAGT373400.038.0507359
GGTATCA130400.035.6074071
TATCACG3450.034.4953232
ACGGTAC6400.031.6076933
ATAACGC6900.031.0418243
ATAGTAC7950.030.6838974
GACCGTT4500.030.4101357
AGAGTAC364600.029.67561310-11
TAGTACT8350.029.214015
CATGGGT28300.028.8023514