FastQCFastQC Report
Fri 10 Feb 2017
SRR3559628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9083880
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1972812.1717702127284815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT927941.021523842234816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579880.6383615811745642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341200.3756104219782736No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252450.2779098799191535No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187010.20587017882226538No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148630.16361951060560026No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136750.1505413986094048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA721300.063.1068081
TATCAAC934050.048.786572
AACGCAG955300.048.782826
ATCAACG942700.048.166053
TCAACGC959400.047.8485374
CAACGCA971200.047.3529435
ACGCAGA1060850.043.912327
CGCAGAG1075950.043.373468
GCAGAGT1143250.040.8097729
GGTATCA333250.040.038061
AGAGTAC1118450.032.0014610-11
CGCATAG13350.030.8142011
GTACATG686700.030.3606951
ACATGGG664850.030.1834473
AGTACTT793200.030.07014512-13
TACATGG694800.029.9410252
CATGGGG263750.029.9107444
TATCACG12350.028.4275932
GAGTACT734100.027.69717412-13
CATGGGT121250.027.4287624
Produced by FastQC (version 0.11.5)