FastQCFastQC Report
Fri 10 Feb 2017
SRR3559627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3960279
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT798712.0168023515514943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377530.9532914221447528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243910.6158909511173329No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144210.36414101127723575No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132800.3353299098371605No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83230.2101619608113469No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60500.15276701464720036No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56320.14221220272612106No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56270.14208594899500768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA359400.054.683011
TATCAAC445050.043.8923382
AACGCAG462200.043.8550386
ATCAACG448100.043.4160273
TCAACGC458650.043.1308024
CAACGCA464150.042.824815
ACGCAGA512600.039.5663267
CGCAGAG520300.039.1294258
CGCATAG7550.037.8970871
GCAGAGT547500.037.2071959
TAACGCA7400.036.1791274
TATCACG6100.036.090972
ACAACGC8250.033.893873
TACAACG8250.032.455272
CATGGGG123400.031.1454524
GGTATCA192950.029.8430941
AGAGTAC535850.028.91179310-11
ACATGGG328400.028.8414573
AGTACTT354000.028.79881512-13
AAACGCA12650.028.2187635