FastQCFastQC Report
Fri 10 Feb 2017
SRR3559626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5804674
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT918871.5829829547705867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434780.7490170851972049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291950.5029567551941763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162590.2801018627402676No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134960.23250229039563633No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93270.1606808582187389No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73380.12641536802928124No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69970.12054079178262207No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63200.10887777677092633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA425400.055.3454321
TATCAAC539800.043.486672
TCAACGC549650.043.178124
AACGCAG559750.043.154056
ATCAACG543800.043.0846373
CAACGCA556350.042.700915
ACGCAGA616850.039.1690527
CGCAGAG630400.038.336918
GCAGAGT663250.036.4112179
GGTATCA227800.032.401051
CATGGGG184800.030.9712724
TAACGCA9200.030.3945644
ACATGGG446900.029.0093
AGAGTAC646400.028.70369310-11
GTACATG477800.028.5497341
CGCATAG9850.028.4481331
AGTACTT397850.028.39235712-13
TACATGG482950.028.153092
GAGTACT358750.027.03372812-13
CATGGGT71750.026.6176074
Produced by FastQC (version 0.11.5)