FastQCFastQC Report
Fri 10 Feb 2017
SRR3559625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632591
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543052.062796689649095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254180.965512683132321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178120.6765957947892399No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101820.38676725704828435No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87790.3334737526642004No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59510.2260510652813141No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.15080200456508436No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.1422932768515884No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30240.11486782413219525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA236750.054.8354531
CGCATAG5950.048.085911
AACGCAG307400.044.3410226
TATCAAC295650.043.875372
ATCAACG298250.043.407313
TCAACGC307050.043.035214
TACAACG5700.042.800732
CAACGCA312650.042.5308045
TATCACG4100.040.636512
ACGCAGA344100.039.5772447
CGCAGAG347250.039.3038988
GCAGAGT364500.037.3948679
ACAACGC6650.036.6814653
CATGGGT41100.034.5971684
GGTATCA126600.032.298621
TAACGCA4750.031.3134445
ACATGGG226600.030.9292833
CATGGGG85250.030.9167484
GTACATG236600.030.710191
TACATGG240400.030.3705252