FastQCFastQC Report
Fri 10 Feb 2017
SRR3559624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6257550
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1403892.2435138352869735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675651.0797356792994064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436930.6982445206190921No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257780.4119503639603359No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201590.322154836956956No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145200.232039696047175No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105810.16909173718148476No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101410.16206023124066127No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93910.15007470975062126No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT78550.12552836173901927No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC76670.12252399101884923No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70450.11258399852977603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA599750.056.0681151
TATCAAC752900.044.629552
AACGCAG774500.044.500176
ATCAACG755600.044.3527373
TCAACGC770850.043.961524
CAACGCA779850.043.5079235
CGCATAG11400.040.7940521
ACGCAGA857950.040.2064557
CGCAGAG867150.039.828238
GCAGAGT912700.037.827499
GGTATCA312700.034.6635551
TATCACG9200.034.2791522
TACAACG10550.033.8408162
CATGGGG187650.031.0072274
ACATGGG565400.030.3151763
TAACGCA12450.030.1053564
GTACATG608950.029.7645281
TACATGG608850.029.7590482
AGAGTAC893350.029.48294810-11
CATGGGT84150.029.1990384
Produced by FastQC (version 0.11.5)