FastQCFastQC Report
Fri 10 Feb 2017
SRR3559622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142777
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT892632.8402587902355148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397771.26566409261618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268950.8557718221814656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151480.4819941090315985No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112120.3567545517865251No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77840.247679043088326No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.20908260433368325No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58860.18728659398996492No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA49470.15740855937280948No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT39780.12657595495957874No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC38950.12393497852377054No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35580.11321197781452517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA374550.054.5650751
AACGCAG468450.044.7693146
TATCAAC460400.044.2553632
ATCAACG467100.043.5867733
CGCATAG5900.043.4510421
TCAACGC475900.043.2933654
CAACGCA481800.042.874345
ACGCAGA518150.040.4751247
CGCAGAG521600.040.242278
GCAGAGT545400.038.4861879
TACAACG5250.037.404352
GGTATCA201900.031.3006461
TAACGCA5700.030.2696725
AGAGTAC537500.029.38850810-11
TATCACG6100.029.26572
AGTACTT360700.029.02340912-13
CATGGGG101650.028.6796114
ACAACGC7300.028.525273
TCACGCA6750.028.2053034
GTACTGG23900.027.68911