FastQCFastQC Report
Fri 10 Feb 2017
SRR3559621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575000
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT562073.568698412698413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252761.604825396825397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176401.1199999999999999No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101910.6470476190476191No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76830.4878095238095238No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51530.3271746031746032No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42230.2681269841269841No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39170.2486984126984127No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37610.23879365079365078No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29400.18666666666666668No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24100.15301587301587302No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT23950.15206349206349207No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19980.12685714285714286No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18870.11980952380952381No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15840.10057142857142858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG3550.055.3141562
CGCATAG3900.053.5171281
GTATCAA241650.052.4151761
ATAACGC4500.043.631183
AACGCAG305000.042.818386
TATCAAC294250.042.810972
TAACGCA4450.042.7844054
ATCAACG301100.041.811953
TCAACGC308750.041.219184
CAACGCA313000.040.7735445
GTATAAC5000.040.550691
AAACGCA5300.040.41315
GTACAAC5500.040.1169931
ACAACGC5000.039.2680633
GACCGTT3650.039.1213577
ACGCAGA335500.038.9612667
CGCAGAG338850.038.6814358
GCAGAGT352150.037.2205129
TTCAACG8600.032.515844
CATGGGG48200.031.60014