FastQCFastQC Report
Fri 10 Feb 2017
SRR3559620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013295
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214202.1138957559249776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104691.033164083509738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76790.7578247203430393No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54670.5395269886854273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43910.4333387611702416No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29440.29053730650995024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24750.24425266087368436No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.1555321994088592No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15610.154051880252049No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14480.14290014260407877No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10890.10747117078442113No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10390.10253677359505375No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10300.10164858210096764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2300.051.8505171
TAACGCA2500.047.596554
TATCACG1400.046.7581442
GTATCAA119200.044.37091
TACAACG2000.038.6817362
GTACAAC3400.038.5828821
TATCAAC143200.036.9030762
AACGCAG149700.036.804096
ATCAACG144550.036.4670873
ATAGTAC3450.036.2147644
TCAACGC148050.036.12744
CAACGCA149600.035.9917075
TCTAACG851.7085906E-435.0060962
ACGCAGA162450.033.878877
CGCAGAG165100.033.4792338
GTACTGG6750.032.6850281
GCAGAGT170200.032.5459569
TACTGGT5700.032.3652842
CATGGGG35000.032.127674
TAGTACT3900.032.0361375
Produced by FastQC (version 0.11.5)