FastQCFastQC Report
Fri 10 Feb 2017
SRR3559619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356413
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT652442.7687845891191403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302451.2835186361643736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206870.8779021334545344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119310.5063204115747112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100790.42772637903457494No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70750.3002444817610495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51090.21681258760667166No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48930.2076461129691612No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42810.18167443482954815No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30150.12794870848191722No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24260.10295309014166873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5150.056.732451
TACAACG4700.053.168342
GTATCAA284600.052.524521
AACGCAG352900.043.4784136
TATCAAC349500.042.6784632
ATCAACG354750.041.8252753
TCAACGC362900.041.4433564
CAACGCA366550.041.1605155
ACAACGC6150.040.6292653
TAACGCA4950.039.66194
ACGCAGA390950.039.216357
CGCAGAG394450.038.973968
GCAGAGT410750.037.4128539
CATGGGT39650.036.911074
ATAGTAC8200.035.5506064
AGATTAC9300.033.2676432
GGTATCA156500.032.8424531
CTAACGC1701.2631062E-831.4962143
CATGGGG78700.031.44734
TAGTACT10050.031.3743365
Produced by FastQC (version 0.11.5)