FastQCFastQC Report
Fri 10 Feb 2017
SRR3559618.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559618.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7162037
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1574392.1982433209993193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT766671.0704636125169418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT484880.677014095291605No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286310.39976057091020334No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217700.30396380247686516No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154900.21627925128004785No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA141000.1968713649482682No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT133000.18570135842638064No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123240.17207395046967783No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC119560.16693574746960957No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109380.15272191417050762No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT109190.15245662651561281No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC102790.14352062129810275No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT91930.1283573374446404No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA90290.12606748610765345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA737450.051.3302761
TATCAAC904000.041.7462962
AACGCAG934100.041.3618746
ATCAACG923350.040.8189243
TCAACGC939200.040.592494
CAACGCA947800.040.2932175
CGCATAG14900.039.2263151
ACGCAGA1024250.037.651687
CGCAGAG1033500.037.372528
TAACGCA10000.036.886974
GCAGAGT1084050.035.629819
TACAACG10750.034.8732342
TACTGGT59350.031.7832762
GTACTGG67850.031.4681241
GGTTCAC61750.029.771646
GACCGTT15650.029.6525177
GGTATCA418500.028.5587981
GGACCGT31300.028.5120356
CATGGGG220150.028.10584
AGTACTT709400.028.02068312-13
Produced by FastQC (version 0.11.5)