FastQCFastQC Report
Fri 10 Feb 2017
SRR3559617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971974
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1039072.6160040322519738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482441.2146101661289828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318850.8027494641203593No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170120.4283008901870959No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138660.34909594070857464No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98660.2483903469660174No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75500.19008180818907677No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71720.1805651295804051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA374500.063.5679471
TATCAAC477900.049.8047522
AACGCAG490050.049.6801576
ATCAACG484500.048.9831853
TCAACGC496250.048.422824
CAACGCA502700.047.801525
GGTATCA173000.044.904411
ACGCAGA546700.044.510447
CGCAGAG556150.043.8290188
GCAGAGT582000.041.8516469
TACAACG7450.035.942152
ACAACGC8050.033.9968533
TATCACG4850.033.126072
AGAGTAC573950.032.33691810-11
CGCATAG8050.031.1059951
CATGGGG123400.030.4704384
TAACGCA7650.030.3305264
AGTACTT422000.030.29041112-13
GTACATG320700.029.6147121
ACATGGG312900.029.5476023
Produced by FastQC (version 0.11.5)