FastQCFastQC Report
Fri 10 Feb 2017
SRR3559615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417714
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1443302.6640387440163877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT687481.2689484900827175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424480.7835038911245592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247740.45727773743685984No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196190.36212690444715245No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142690.2633767673967286No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112080.2068769226282524No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA108710.20065658689255284No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108100.1995306507504826No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT89790.16573410851883286No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC81930.15122614445871452No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT73420.13551841237835735No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC62230.11486394446070795No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG57560.10624407268453079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA638850.053.6595571
CGCATAG12250.046.2344471
TACAACG9100.044.463442
TATCAAC788950.043.358912
AACGCAG818200.043.112326
ATCAACG806850.042.375983
TCAACGC825700.041.963384
CAACGCA833000.041.7027665
ACGCAGA894500.039.3683667
CGCAGAG904250.039.0231978
TAACGCA9400.037.9747354
GCAGAGT946750.037.2588589
TATCACG10600.037.048752
TCACGCA11150.034.1488844
GTATAAC14400.031.4651131
ATAGTAC19150.031.0672364
GTACAAC16900.029.9854371
ACAACGC13700.029.964013
AGTACTT622450.029.89429312-13
TAGTACT20450.029.6741495
Produced by FastQC (version 0.11.5)