FastQCFastQC Report
Fri 10 Feb 2017
SRR3559612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832967
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479791.6935954425166266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232250.8198118792064998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153930.5433526052368418No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89430.31567610918164596No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89020.3142288632377292No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56480.1993669534449219No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43090.1521020188374944No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35610.12569860503140348No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34700.12248642500953946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA4600.049.1461374
GTATCAA230900.048.9595261
CGCATAG5800.048.316191
AACGCAG295500.039.259836
TATCAAC286750.039.2191932
ATCAACG287250.038.978333
TCAACGC291550.038.9748044
CAACGCA296550.038.4982225
ACGCAGA328400.035.2904437
CGCAGAG333200.034.9070438
GTATAAC8500.032.9686971
GCAGAGT353400.032.911799
CATGGGG88700.030.9871774
ACATGGG224050.029.4210683
GTACATG242850.028.8730071
TACAACG6400.028.821862
TACATGG243650.028.646522
TATCACG5500.028.1287822
GGTATCA134750.028.0975061
AGAGTAC342900.026.91879310-11
Produced by FastQC (version 0.11.5)