FastQCFastQC Report
Fri 10 Feb 2017
SRR3559611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000687
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT805392.684018693052624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382511.2747414175487146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266960.8896629338548139No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130340.43436719657865014No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81710.27230430897991026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61310.20431987741473867No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59790.19925437074909844No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52210.17399348882439256No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC44820.14936579523289167No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44700.14896588681192008No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38460.12817064892139698No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33440.11144114664408517No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31850.1061423600662115No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA30490.1016100646285334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA356600.060.129341
CGCATAG5900.048.4971851
TATCAAC447400.047.9087032
AACGCAG464300.047.7581756
ATCAACG456750.046.828193
TCAACGC467850.046.442034
CAACGCA474300.045.8857275
ACGCAGA510950.043.444417
CGCAGAG513850.043.2455378
GCAGAGT538700.041.1512379
GGTATCA173150.039.0062981
CATGGGT60550.036.4541364
TACAACG4950.033.6604162
TCAAACG5100.031.4978313
AGAGTAC533350.031.401710-11
TAACGCA5700.031.3136335
TAGTACT9800.030.9621525
AGTACTT371850.030.36845612-13
GTACTGG21250.030.2964761
AGATTAC11750.029.8800142
Produced by FastQC (version 0.11.5)