FastQCFastQC Report
Fri 10 Feb 2017
SRR3559610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813963
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592882.106921803875886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272870.9697000280387481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193580.6879266003142188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93930.33379969814812777No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.22644220979451402No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47120.16745067365846672No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42060.14946891625796074No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35750.12704502511227048No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35460.12601445008338774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA267400.057.9183351
AACGCAG348600.045.7399866
TATCAAC337750.045.736162
ATCAACG339450.045.3946723
TCAACGC347800.044.9719734
CAACGCA352650.044.471575
TATCACG3700.041.8140262
CGCATAG5150.041.670971
ACGCAGA385950.041.2518737
CGCAGAG391550.040.8594178
GCAGAGT411450.038.8543139
TAACGCA5700.038.61964
GGTATCA135000.035.635111
CATGGGT38550.031.0207714
AGAGTAC404600.030.2847910-11
TACAACG5550.030.0203252
CATGGGG92150.029.7636724
AGTACTT265800.029.66998112-13
ACATGGG249500.028.7579333
GTACATG268200.028.1615241