FastQCFastQC Report
Fri 10 Feb 2017
SRR3559608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191436
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT642381.2373840301604413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299060.5760641179049496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195830.37721740189034403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109650.21121323656884144No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82150.15824138061222368No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60400.11634545817380779No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56490.10881382338143049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA333350.054.6762961
AACGCAG432700.043.3672946
TCAACGC425500.043.2897154
ATCAACG420200.043.1844253
CAACGCA429200.042.8888055
TATCAAC424450.042.870762
ACGCAGA480250.039.0115177
CGCAGAG490300.038.308958
GCAGAGT523300.035.8703969
CGCATAG8400.034.0583841
TATCACG7500.032.5291632
CATGGGT72100.032.429534
ACATGGG361900.030.7916223
GTACATG386450.030.3987241
GGTATCA178800.030.3011041
TACATGG390400.030.102852
CATGGGG147800.029.5464294
AGAGTAC505550.028.39165710-11
AGTACTT316600.027.55962812-13
GAGTACT286200.026.21486512-13
Produced by FastQC (version 0.11.5)