FastQCFastQC Report
Fri 10 Feb 2017
SRR3559606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935299
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437842.262389429230315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207591.0726507893612305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144740.7478947697487571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72000.3720355355942415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61780.31922715818072556No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60690.3135949535446461No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43730.22595991627133585No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42540.2198109956135977No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32780.16937951189971162No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31840.16452238129612012No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC31490.16271387522031477No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC29600.15294794241096596No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28130.1453522168925835No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC27180.14044341468682617No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26830.13863490861102082No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA25010.12923067701683305No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT22560.11657113448619567No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21520.1111972878609455No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20740.10716690289200789No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20130.10401493515989002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4550.051.0908741
TAACGCA4750.047.5985764
TACAACG3400.047.255912
GTATCAA261300.043.4555971
TATCACG3550.041.9066472
TCACGCA3900.039.6654784
GTATAAC6200.039.4168851
ATAGTAC6350.037.4791954
ATAACGC6250.037.126893
AACGCAG319500.036.741786
TACTGGT15600.036.6199652
TATCAAC311800.036.3380932
ACAACGC4450.036.100043
ATCAACG315600.035.8195883
TCAACGC322200.035.7506334
CAACGCA323800.035.7026065
GTACTGG18800.034.5588071
ACGCAGA345600.034.0186587
CGCAGAG349550.033.7023248
AGATTAC9100.032.6963962
Produced by FastQC (version 0.11.5)