FastQCFastQC Report
Fri 10 Feb 2017
SRR3559605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4587720
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1153252.513775906114584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528191.15131263459845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346090.7543834410120931No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183370.39969745320115435No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143120.31196324099988665No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101850.2220057021788601No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82800.18048180795689361No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82570.1799804696014578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA417050.062.886331
TATCAAC533450.049.0168652
AACGCAG544700.049.011446
ATCAACG540700.048.3165973
TCAACGC550600.047.7936334
CAACGCA558850.047.131185
ACGCAGA603600.044.1199347
CGCAGAG611750.043.6294068
GGTATCA192450.042.7922331
GCAGAGT644200.041.394749
CGCATAG7300.032.6758041
AGAGTAC632550.031.95175410-11
TACAACG8150.030.6669732
AGTACTT440300.030.34991312-13
CATGGGG151350.030.1117274
TAACGCA6200.029.7481024
GTACATG377500.029.050521
ACATGGG371650.028.9436683
TACATGG383350.028.9354112
GAGTACT414750.027.99487312-13
Produced by FastQC (version 0.11.5)