FastQCFastQC Report
Fri 10 Feb 2017
SRR3559604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5053038
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1716223.3964122177589005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT781631.546851616789741No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504520.9984488539369781No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282470.5590102429469163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198440.39271424438130087No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140170.27739747850698926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126480.2503048661023329No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118180.23387910401623738No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88250.17464741013228083No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71920.14233021797975792No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC70010.1385503136924757No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT66460.1315248371375794No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC54130.10712367490606642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA663500.058.798711
CGCATAG8950.057.3112641
TATCAAC821750.047.3591962
AACGCAG847250.047.162486
ATCAACG840150.046.264623
TCAACGC862750.045.6250884
CAACGCA873400.045.1845555
ACGCAGA929650.042.9625787
CGCAGAG938800.042.6262368
GCAGAGT978900.040.8679249
GACCGTT10100.039.4681367
TACAACG10650.038.553672
GGACCGT16900.034.8534666
GGTATCA333750.034.7586481
TATCACG8500.034.3038942
TTAGGAC17750.032.178523
CATGGGG167550.031.639274
AGAGTAC965200.031.4096210-11
AGTACTT684300.031.22752412-13
ACAACGC14000.030.5983243
Produced by FastQC (version 0.11.5)