FastQCFastQC Report
Fri 10 Feb 2017
SRR3559603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240445
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT956902.256602785792529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448511.0576955956273457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293990.6932998777250973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165340.3899119078304282No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134410.31697144993037285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97960.23101349032943475No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72230.17033589635050095No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70980.16738809252330827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43940.10362120013347656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA369300.060.1846771
AACGCAG473700.047.865346
TATCAAC466000.047.6444132
ATCAACG471100.046.992893
TCAACGC480000.046.6421554
CAACGCA485550.046.1825335
ACGCAGA525200.043.2057537
CGCAGAG532800.042.5676238
GCAGAGT561650.040.306929
GGTATCA181300.037.860241
TAACGCA5800.036.9283684
CGCATAG7450.032.0191351
AGAGTAC550500.030.87760510-11
AGTACTT387800.029.48075712-13
CATGGGG128000.028.5858274
GTACATG336550.028.3692341
GTACTGG30050.028.1805671
ACATGGG325850.028.1730463
TACATGG341900.027.987212
GAGTACT358400.027.7903212-13
Produced by FastQC (version 0.11.5)