FastQCFastQC Report
Fri 10 Feb 2017
SRR3559602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900661
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333561.7549684030976591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159720.8403392293523148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111390.5860592709588928No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68670.3612953598774321No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.3440382056558218No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55590.29247719609125455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40750.21439909589348127No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36690.19303810621673195No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.13826768687314572No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.12474607518121326No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22100.11627533789560578No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21580.11353944759217977No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20660.10869902628611834No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG19280.10143839432702623No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19260.10133316777689447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA3900.048.816534
GTATCAA197050.041.1143761
CGCATAG3550.038.623371
TACAACG4600.037.5127452
CATGGGT29250.035.5953874
AACGCAG243750.034.3189246
TATCAAC234650.034.182832
ATCAACG235800.033.860263
TCAACGC240600.033.8276634
CAACGCA244500.033.3367465
GTATAAC6600.032.5169071
CATGGGG59500.032.1973724
ACAACGC5250.031.7307453
ACGCAGA267000.031.2859137
CGCAGAG269600.030.9621268
GTACAAC7350.030.8210161
GCAGAGT279350.029.9027679
TATCACG3250.029.293772
ACATGGG188200.028.672743
GTACATG213200.027.1508061
Produced by FastQC (version 0.11.5)