FastQCFastQC Report
Fri 10 Feb 2017
SRR3559601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523040
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414611.6432953896886295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199500.7907127909188915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137690.5457305472763017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79500.3150960745767011No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.28715359249159744No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.1850545373834739No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.1239774240598643No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27780.11010526983321707No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27200.10780645570422982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA187500.053.7425961
TAACGCA4650.047.340654
AACGCAG241650.043.1599126
TATCAAC235200.042.88062
CGCATAG4450.042.8768771
ATCAACG234100.042.87453
TCAACGC238500.042.6073884
CAACGCA242200.042.1530085
ACGCAGA267350.038.8774837
CGCAGAG273450.038.162528
GCAGAGT287450.036.2831579
GGTATCA100600.032.7170641
GTATAAC8100.030.9170071
ACATGGG189450.030.1482453
CATGGGG80100.030.0820854
GTACATG198700.029.7378121
TACATGG201050.029.417842
AGAGTAC277650.028.96034810-11
TATCACG3300.028.8492492
CATGGGT28800.028.7149284