FastQCFastQC Report
Fri 10 Feb 2017
SRR3559600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115728
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239822.1494486111310285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112171.0053525590466494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80710.7233841939971032No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54190.48569185321153546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44970.40305522492937346No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.2546319533076162No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22780.20417162605939798No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.15828230536474838No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.14412114780663388No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12840.11508181205455094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1950.064.0818942
TAACGCA2750.054.0854074
GTATCAA114450.047.4910281
CGCATAG2600.045.8447041
TCACGCA3250.043.9339944
GTATAAC3750.042.9106451
GTATCAC4400.039.280581
AACGCAG144850.038.854876
TACAACG1850.038.597592
TATCAAC142950.037.9214442
ATCAACG143300.037.697493
TCAACGC146600.037.5388154
CAACGCA147750.037.206375
ATAACGC4900.036.4248663
ATCACGC3800.036.0094953
CGCAGAG163600.034.729058
ACGCAGA162050.034.6573837
AAACGCA3200.033.4653475
GCAGAGT172600.032.849219
CATGGGG32250.030.4387634