FastQCFastQC Report
Fri 10 Feb 2017
SRR3559597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150035
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT694742.2054993039759876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329411.045734412474782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211000.6698338272431894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127670.40529708400065395No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110790.3517103778211988No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74220.23561642965871807No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53950.17126793829274914No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.15949029137771487No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42820.1359349975476463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA287500.055.851841
CGCATAG5300.049.501211
AACGCAG373800.044.343346
TATCAAC365750.043.7469942
ATCAACG369750.043.2521483
TCAACGC378550.042.938224
CAACGCA381000.042.8182645
ACGCAGA414350.039.9887247
CGCAGAG420650.039.587838
GCAGAGT444150.037.4932379
GGTATCA151600.034.8869821
TACAACG5800.033.855382
CATGGGG96400.030.6736834
ACATGGG266000.030.5083943
CATGGGT43900.030.3577884
GTACATG280450.030.0205211
TACATGG281500.029.9102332
AGAGTAC434200.029.89817210-11
GTATAAC7600.029.0286671
TAACGCA4950.028.8464684