FastQCFastQC Report
Fri 10 Feb 2017
SRR3559593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6079048
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1228782.0213362355421443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT554760.9125771008881653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373990.6152114607418794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197410.324738347188573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145920.2400375848323619No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109130.17951824035605576No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92130.15155333532487325No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82230.13526789063024341No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74790.12302913219306708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA549750.055.9470831
AACGCAG705400.044.3400736
TATCAAC691700.044.328472
ATCAACG700300.043.7273563
TCAACGC710600.043.5037964
CAACGCA719600.043.0427255
ACGCAGA778100.040.128447
CGCAGAG789750.039.6046188
GCAGAGT832450.037.5516859
GGTATCA289150.031.8141161
TATCACG9000.030.4127482
CGCATAG10700.030.087571
AGTACTT513400.029.72597712-13
CATGGGG197100.029.611854
AGAGTAC814600.029.3067810-11
ACATGGG513000.027.938473
CATGGGT85800.027.8061124
GAGTACT477150.027.5888112-13
GTACATG550300.027.4634821
TAACGCA8700.027.3541954