FastQCFastQC Report
Fri 10 Feb 2017
SRR3559592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730782
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1004883.679825046451896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467601.7123300212173655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309941.134986242036164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167340.6127915007496021No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131230.48055831626252116No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92260.3378519413120491No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73890.27058183333565256No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68470.2507340388211142No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47080.17240482762812998No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC36700.13439373776449381No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35100.1285346102325268No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31200.11425298687335717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA375950.059.42261
TACAACG4850.058.893312
CGCATAG5650.052.7758871
TATCAAC471400.047.4892922
AACGCAG485900.047.191066
ATCAACG481950.046.354823
TCAACGC494550.045.8234444
CAACGCA501200.045.227325
ACGCAGA537100.042.6703347
CGCAGAG541900.042.3582468
ACAACGC7150.041.605523
GCAGAGT565400.040.5766459
TAACGCA5600.038.2473564
GGTATCA185400.037.0880131
TATCACG4700.036.7169532
GTACAAC9000.036.444681
AGATTAC11250.034.910652
GACCGTT6500.032.952177
TCACGCA5900.032.2689554
GGACCGT9350.031.1802356