FastQCFastQC Report
Fri 10 Feb 2017
SRR3559591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783010
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT873033.1369991484040662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408941.4694162076313058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273410.9824255033219428No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152500.5479678477619556No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124450.44717769609164176No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88730.3188274566027431No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67190.24142924387623474No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64020.23003869910636324No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60760.21832476347551752No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49620.17829616135048024No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC42690.15339506505546152No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT40780.14653199233922984No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC35500.12755972849540606No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA32390.11638477763285075No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29850.10725796888979917No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT29450.10582067617435797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6000.054.659321
GTATCAA381000.052.3352241
AACGCAG472700.043.156926
TATCAAC463000.042.8979342
ATCAACG472700.041.9235083
TCAACGC484400.041.549564
TACAACG5600.041.438842
CAACGCA487300.041.326715
ACGCAGA519450.039.2728427
CGCAGAG523000.039.0076688
ATAGTAC8950.037.8896334
GCAGAGT547100.037.2132389
TATCACG5150.035.816692
CATGGGT37700.033.6129654
CATGGGG82500.033.099954
ACAACGC6950.032.5287483
GTACAAC9300.032.0582541
AGATTAC12000.031.734362
TAGTACT11450.030.6559755
GTATAAC7100.030.2340971
Produced by FastQC (version 0.11.5)