FastQCFastQC Report
Fri 10 Feb 2017
SRR3559590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529016
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT654341.8541712477359127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322350.9134274256619975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204830.5804167507316488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119260.3379412278096784No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100680.2852919907418952No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70640.20016911229645884No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58080.1645784547307238No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50330.14261765886014685No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47630.13496680094394584No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42340.11997678673035203No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38870.11014401748249371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA310250.052.4804081
TATCAAC385250.042.1981772
AACGCAG399100.041.919436
ATCAACG388850.041.66213
TCAACGC395600.041.507684
CAACGCA400150.041.110635
ACGCAGA439000.038.163657
CGCATAG6100.038.1172941
CGCAGAG446300.037.526098
GCAGAGT468900.035.6920369
TATCACG5650.033.7021182
CATGGGG106100.032.466754
GGTATCA172850.031.1462421
TAACGCA5350.030.025184
GGACCGT10950.029.8834256
TACTGGT22850.029.4271282
ACATGGG286800.028.8759163
AGAGTAC457100.027.9644310-11
AGTACTT299950.027.71906712-13
TACAACG5800.027.7007452