FastQCFastQC Report
Fri 10 Feb 2017
SRR3559588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7992758
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2127072.661246593478747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1104551.3819384998269684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT662440.8288002714457262No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374860.4689995618533678No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332490.41598907410933744No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233550.2922020158748707No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA176890.2213128434515345No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165230.206724637478077No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161230.20172010712697672No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT138540.17333190871036006No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC104340.1305431742084522No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT83380.10431943516868647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA915500.053.913431
AACGCAG1179200.042.7896776
CGCATAG13550.042.6940421
TATCAAC1156450.042.6861042
ATCAACG1177250.041.864453
TCAACGC1203450.041.3781784
CAACGCA1212600.041.1150135
ACGCAGA1293000.039.000657
CGCAGAG1301050.038.8190278
GCAGAGT1359900.037.10859
CATGGGT142700.034.270614
CATGGGG231950.031.831084
GGTATCA499650.031.547581
TACAACG13250.030.5377942
AGAGTAC1343850.029.5030110-11
AGTACTT903100.029.48172612-13
TATCACG12000.029.256032
ACATGGG788800.028.608163
GTACTGG52150.028.4760571
GTACATG857300.027.8128051
Produced by FastQC (version 0.11.5)