FastQCFastQC Report
Fri 10 Feb 2017
SRR3559587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822896
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1262233.301763898363963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637761.6682640594983489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439861.1505936860432509No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337840.8837279381913606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274090.7169695435083768No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190460.4982086878638603No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109560.28658901523870906No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108040.2826129719458756No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA90420.23652225956447676No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58760.15370546308348434No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55910.14625038190942155No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51580.1349238901607577No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC51010.13343287392594516No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49890.13050315781543625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49730.13008462694250642No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG47870.125219205544697No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42070.11004746140099025No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.10455424369378606No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39760.10400492192306565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13650.068.6430051
TAACGCA10100.059.5203364
TACAACG8650.058.488192
TATCACG7350.051.827232
GTATCAA538900.050.0874861
ATAGTAC20250.047.616274
ATAACGC13400.047.083253
AGATTAC24800.046.8002622
GCATAGT21050.045.2411082
GTATAAC13650.044.4419441
TAGTACT22250.043.3163155
ACAACGC11800.042.8748173
GATTACT27500.042.6382033
AGTGTAC20950.041.763673
TCACGCA9350.041.3777734
TATCAAC663800.040.4842262
ATCAACG680950.039.4646153
TCAACGC702850.039.056384
CAACGCA709150.038.943375
AACGCAG729700.038.866796
Produced by FastQC (version 0.11.5)