FastQCFastQC Report
Fri 10 Feb 2017
SRR3559586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459385
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400772.746156771516769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200011.3705088102180027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184551.264573775939865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166651.1419193701456436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84930.5819574683856555No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76930.527139856857512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71190.48780822058606876No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36250.24839230223690115No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.23064510050466464No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT31960.2189963580549341No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.19515069704019158No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.1617804760224341No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22370.15328374623557184No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21690.14862424925567963No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19620.13444019227277243No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17910.12272292780863173No Hit
CTGCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT17360.11895421701607184RNA PCR Primer, Index 33 (95% over 22bp)
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.1073054745663413No Hit
GCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT15550.10655173240782932RNA PCR Primer, Index 33 (95% over 24bp)
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.10634616636459879No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.1049757260763952No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG14940.10237188952880838No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT14860.10182371341352693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6450.076.358271
TAACGCA5500.066.018534
TACAACG3400.061.275642
GTATAAC6650.058.7075041
ATAGTAC8800.056.8192254
TAGTACT9250.055.3117375
ATAACGC7150.053.2810333
GCATAGT9550.052.980282
TATCACG2900.051.3145752
ACAACGC4300.051.2191053
TCACGCA3100.049.9241144
AGATTAC9900.048.7021942
ATCACGC3500.044.21853
GTATCAA207050.044.0350531
GATTACT11150.043.2423063
CATAGTA11750.043.060573
GTACAAC5350.042.661071
GTATCAC4800.041.2928281
ACGCATA1800.040.0415341
AAACGCA6900.038.799315
Produced by FastQC (version 0.11.5)